国内政策【切换】 国际政策

Use of active extracts of poplar buds against Penicillium italicum and possible modes of action

Abstract：
Antifungal components, from poplar buds active fraction (PBAF) against Penicillium italicum, the causal agent of blue mold in citrus fruits, were identified and possible action modes were investigated.
Pinocembrin, chrysin and galangin were determined as active components in PBAF, using HPLC and HPLC–MS analysis. The antifungal activity is stable at temperatures ranging from 4
C to 100
C and
pH levels ranging from 4 to 8. In the presence of PBAF, the hyphae become shriveled, wrinkled and the cell membrane became seriously disrupted. Further investigation on cell permeability, nucleic acid
content and alkaline phosphatase suggest that the cell membrane might be the target. Mycelial oxygen consumption and the respiration-related enzymatic activity of succinate dehydrogenase, malate dehydrogenase and ATPase were all inhibited by PBAF. We propose that PBAF is a potentially useful alternative for blue mold control and may act against P. italicum by interfering with respiration and disrupting the cell membrane.
Keywords:Poplar buds; Active constituents; P. italicum; Mode of action; Respiration
摘要：对柑橘青霉病病原菌，意大利青霉表现抗菌作用的杨树芽中提取物中的抗真菌活性成分鉴定出来，并且调查了其可能的作用机制。采用HPLC和HPLC-MS分析，杨树芽活性组分中的抗真菌成分确定为松鼠素、白杨素、高良姜素。抗真菌活性在4-100度下，pH?4-8条件下稳定。在存在杨树芽活性组分时，真菌扭曲、收缩、细胞膜结构严重破坏。进一步分析细胞通透性、核酸含量、碱性磷酸酶活性发现细胞膜可能是作用靶标。菌丝的呼吸好样和呼吸代谢相关酶琥珀酸脱氢酶、苹果酸脱氢酶和ATP酶均被杨树芽活性组分抑制。我们认为杨树芽活性组分是控制柑橘青霉病的潜在替代方法，其可能通过干扰呼吸和破坏细胞膜结构抑制意大利青霉。

1、浏览PDF格式全文需要使用软件－－Abode Acrobat(由于软件较大并常见,我站不提供下载)

2、下载论文全文请点击鼠标右键“另存为”或使用断点续传软件下载(651KB)